报告题目：Systems biology of plant signaling response to viral infection
报  告 人：Prof. Kristina Gruden
Department of Biotechnology and Systems Biology
National Institute of Biology, Ljubljana, Slovenia
报告时间：10月14日 下午 14:00-15:00
报告地址：闵行校区生物药学楼1号楼105会议室
联  系 人：杨立桃 yylltt@sjtu.edu.cn

Abstract：
The interaction between plants and pathogens initiates a complex signalling network, resulting in massive changes of the gene activity and extensive reprogramming of the cell metabolism. We constructed plant defence signalling network topology describing the biosynthesis, signal transduction and transcriptional regulation leading to activation of effector molecules of crucial phytohormones involved in plant defence: salicylic acid (SA), jasmonic acid (JA) and ethylene (ET). Manually constructed network for model plant Arabidopsis thaliana was complemented with a new automated approach for information extraction from literature, named Bio3graph. To merge all background information available we have extended the network with available protein-protein interaction, transcriptional regulation and miRNA regulation data. Subsequently, we transferred the system from model plant to potato (Solanum tuberosum L.) - Potato virus Y (PVY) interaction. Potato is the world's most widely grown tuber crop and the fourth largest food crop and PVY is and an important potato pathogen worldwide. Kinetic analysis of the whole potato transcriptome in 11 different time points after PVY infection was performed. To study the effect of phytohormones, the expression profiles of two mutants deficient in SA and JA signalling were used in the study. Transcriptomic data was complemented with measurements of photosynthetic activity, proteomics and measurments of callose deposition. Novel components for plant defence were identified and are being studied in detail with functional analysis.